研究業績

原著論文

Bashir K, Ishimaru Y, Itai RN, Senoura T, Takahashi M, An G, Oikawa T, Ueda M, Sato A, Uozumi N, Nakanishi H, Nishizawa NK. Iron deficiency regulated OsOPT7 is essential for iron homeostasis in rice. Plant Mol Biol. (2015) 88:165-76 doi: 10.1007/s11103-015-0315-0

Nozoye T, Kim S, Kakei Y, Takahashi M, Nakanishi H, Nishizawa NK. Enhanced levels of nicotianamine promote iron accumulation and tolerance to calcareous soil in soybean. Bioscience, Biotechnology, and Biochemistry (2014) 78:1677-1684

Nozoye T, Tsunoda K, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK. Rice nicotianamine synthase localizes to particular vesicles for proper function. Plant Signal Behav. (2014) doi : 10.4161/psb.28660

Nozoye T, Nagasaka S, Bashir K, Takahashi M, Kobayashi T, Nakanishi H, Nishizawa NK. Nicotianamine synthase 2 localizes to the vesicles of iron-deficient rice roots, and its mutation in the YXXφ or LL motif causes the disruption of vesicle formation or movement in rice. Plant J. (2014) 77:246-260.

Kato S., Wachi T., Yoshihira K., Nakagawa T., Ishikawa A., Takagi D., Tezuka A., Yoshida H., Yoshida S., Sekimoto H., Takahashi M. Rice (Oryza sativa L.) roots have iodate reduction activity in response to iodine. Frontiers in plant physiol. (2013) doi:10.3389/fpls.2013.00227

Masuda H., Kobayashi T., Ishimaru Y., Takahashi M., Aung M.S., Nakanishi H., Mori S., Nishizawa N.K. Iron-biofortification in rice by the introduction of three barley genes participated in mugineic acid biosynthesis with soybean ferritin gene. Frontiers in plant physiol. (2013) doi:10.3389/fpls.2013.00132

Iwai T., Takahashi M., Oda K., Terada Y., Yoshida K.T. Dynamic changes in the distribution of minerals in relation to phytic-acid accumulation during rice seed development. Plant Physiol. (2012) 160 : 2007-2014

Suzuki M., Bashir K., Inoue H., Takahashi M., Nakanishi H., Nishizawa NK. Accumulation of starch in Zn-deficient rice. Rice 5 : 1-9. (2012)

Masuda H., Ishimaru Y., Aung M. S., Kobayashi T., Kakei Y., Takahashi M., Higuchi K., Nakanishi H., Nishizawa N. K. Iron biofortification in rice by the introduction of multiple genes involved in iron nutrition. Scientific Reports 2 : 1-7. (2012)

Nozoye T., Nagasaka S., Kobayashi T., Takahashi M., Sato Y., Sato Y., Uozumi N., Nakanishi H., Nishizawa NK. Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants. J. Biol. Chem. 286 : 5446-5454 (2011)

Ishimaru Y, Masuda H, Bashir K, Inoue H, Tsukamoto T, Takahashi M, Nakanishi H, Aoki N, Hirose T, Ohsugi R, Nishizawa NK. Rice metal-nicotianamine transporter, OsYSL2, is required for long distance transport of iron and manganese. 
Plant J. 62 : 379-90. (2010)

Takahashi, M., Nozoye, T., Kitajima. N., Fukuda, N., Hokura, A., Terada, Y., Nakai, I., Ishimaru, Y., Kobayashi, T., Nakanishi, H., Nishizawa, NK. In vivo analysis of metal distribution and expression of metal transporters in rice seed during germination process by microarray and X-ray Fluorescence Imaging of Fe, Zn, Mn, and Cu. Plant Soil 325: 39-51. (2009)

Aoyama T, Kobayashi T, Takahashi M, Nagasaka S, Usuda K, Kakei Y, Ishimaru Y, Nakanishi H, Mori S, Nishizawa NK. OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.
 Plant Mol. Biol. 70: 681-692. (2009)

Kobayashi, T., Nakanishi I. R., Ogo, Y., Kakei, Y., Nakanishi, H., Takahashi, M., Nishizawa, NK., The rice transcription factor IDEF1 is essential for the early response to iron deficiency and induces vegetative expression of late embryogenesis abundant genes. Plant J. 60: 948-961. (2009)

Nagasaka S, Takahashi M, Itai RN, Bashir K, Nakanishi H, Mori S, Nishizawa NK. Time course analysis of gene expression over 24 hours in Fe-deficient barley roots.
 Plant Mol. Biol. 69: 621-631. (2009)

Inoue H, Kobayashi T, Nozoye T, Takahashi M, Kakei Y, Suzuki K, Nakazono M, Nakanishi H, Mori S, Nishizawa NK. Rice OsYSL15 is an iron-regulated iron(III)-deoxymugineic acid transporter expressed in the roots and is essential for iron uptake in early growth of the seedlings. J. Biol. Chem. 284: 3470-3479. (2009)

Kakei Y, Yamaguchi I, Kobayashi T, Takahashi M, Nakanishi H, Yamakawa T, Nishizawa NK. A highly sensitive, quick, and simple quantification method for nicotianamine and 2′-deoxymugineic acid from minimum samples using LC/ESI-TOF-MS achieves functional analysis of these components in plants. 
Plant Cell Physiol. 50: 1988-1993. (2009)

Masuda H, Usuda K, Kobayashi T, Ishimaru Y, Kakei Y, Takahashi M, Higuchi K, Nakanishi H, Mori S, Nishizawa NK. Overexpression of the barley nicotianamine synthase gene HvNAS1 increases iron and zinc concentrations in rice grains. 
Rice 2: 155-166.(2009)

K. Usuda., Y. Wada., Y. Ishimaru., T. Kobayashi., M. Takahashi, H. Nakanishi., Y. Nagato., S. Mori and N. K. Nishizawa. Genetically engineered rice containing larger amounts of nicotianamine to enhance the antihypertensive effect. Plant Biotechnol. J., 7:87-95 (2008)

Kobayashi T, Nakanishi H, Takahashi M, Mori S, Nishizawa NK Generation and field trials of transgenic rice tolerant to Iron Deficiency. Rice 1: 144-153.(2008)

H. Masuda., M Suzuki., K. C. Morikawa., T. Kobayashi. H. Nakanishi. M. Takahashi, M. Saigusa., S. Mori., N. K. Nishizawa. Increase in Iron and Zinc Concentrations in Rice Grains Via the Introduction of Barley Genes Involved in Phytosiderophore Synthesis, Rice 1:100–108 (2008)

Inoue H., Takahashi M., Kobayashi T., Suzuki M., Nakanishi H., Mori S. and Nishizawa N.K. Identification and localisation of the rice nicotianamine aminotransferase gene OsNAAT1 expression suggests the site of phytosiderophore synthesis in rice. Plant Mol. Biol., 66: 193-203 (2008)

Ogo Y. Kobayashi T., Nakanishi Itai R., Nakanishi H., Kakei Y., Takahashi M., Toki S., Mori S., and Nishizawa N.K. A novel transcription factor IDEF2 that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants. J. Biol. Chem., 283: 13407-13417 (2008)

Suzuki M., Tsukamoto T., Inoue H., Watanabe S., Matsuhashi S., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Deoxymugineic acid increases Zn translocation in Zn-deficient rice plants. Plant Mol. Biol., 66: 609-617 (2008)

Suzuki M., Morikawa C.K., Nakanishi H., Takahashi M., Saigusa M., Mori S. and Nishizawa N.K. Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil. Soil Sci. Plant Nutr., 54: 77-85 (2008)

Ishimaru Y., Suzuki M., Ogo Y., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Synthesis of nicotianamine and deoxymugineic acid is regulated by OsIRO2 in Zn excess rice plants. Soil Sci. Plant Nutr., 54: 417-423 (2008)

Kobayashi T., Yoshihara T., Nakanishi Itai R., Nakanishi H., Takahashi M., Mori S. and Nishizawa N.K. Promoter analysis of iron-deficiency-inducible barley IDS3 gene in Arabidopsis and tobacco plants. Plant Physiol. Biochem., 45:262-269 (2007)

Ishimaru Y., Kim S., Tsukamoto T., Oki H., Kobayashi T., Watanabe S., Matsuhashi S., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Mutational reconstructed ferric chelate reductase confers enhanced tolerance in rice to iron deficiency in calcareous soil. Proc. Nat. Acad. Sci. USA, 104:7373-7378 (2007)

Ishimaru Y., Masuda H., Suzuki M., Bashir K., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Overexpression of the OsZIP4 zinc transporter confers disarrangement of zinc distribution in rice plants. J. Exp. Bot., 58: 2909-2915 (2007)

Kobayashi T., Ogo Y., Itai R.N., Nakanishi H., Takahashi M., Mori S. and Nishizawa N.K. The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants. Proc. Nat. Acad. Sci. USA, 104: 19150-19155 (2007)

Nozoye T., Inoue H., Takahashi M., Ishimaru Y., Nakanishi H., Mori S. and Nishizawa N.K. The expression of iron homeostasis-related genes during rice germination. Plant Mol. Biol., 64: 35-47 (2007)

Wada Y., Yamaguchi I., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Highly sensitive quantitative analysis of nicotianamine using LC/ESI-TOF-MS with an internal standard. Biosci. Biotechnol. Biochem., 71: 435-441 (2007)

Ogo Y., Nakanishi Itai R., Nakanishi H., Kobayashi T., Takahashi M., Mori S. and Nishizawa N.K. The rice bHLH protein OsIRO2 is an essential regulator of the genes involved in Fe uptake under Fe-deficient conditions. Plant J., 51: 366-377 (2007)

Suzuki M.§, Takahashi M.§, Tsukamoto T., Watanabe S., Matsuhashi S., Yazaki J., Kishimoto N., Kikuchi S., Nakanishi H., Mori S. and Nishizawa N.K. Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley. Plant J., 48: 85-97(2006) (§ These authors contributed to this work equally)

Wada Y., Kobayashi T., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Metabolic engineering of Saccharomyces cerevisiae producing nicotianamine: Potential for industrial biosynthesis of a novel antihypertensive substrate. Biosci. Biotechnol. Biochem., 70: 1408-1415 (2006)

Ogo Y., Nakanishi Itai R., Nakanishi H., Inoue H., Kobayashi T., Suzuki M., Takahashi M., Mori S. and Nishizawa N.K. Isolation and characterization of IRO2, a novel iron-regulated bHLH transcription factor in graminaceous plants. J. Exp. Bot., 57: 2867-2878 (2006)

Bashir K., Nagasaka S., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Cloning and characterization of deoxymugineic acid synthase genes from graminaceous plants. J. Biol. Chem., 281: 32395-32402 (2006)

Ishimaru Y., Suzuki M., Tsukamoto T., Suzuki K., Nakazono M., Kobayashi K., Wada Y., Watanabe S., Matsuhashi S., Takahashi M., Nakanishi N., Mori S. and Nishizawa N.K. Rice plants take up iron as an Fe3+-phytosiderophore and as Fe2+. Plant J., 45: 335–346 (2006)

Kim S.§, Takahashi M.§, Higuchi K., Tsunoda K., Nakanishi H., Yoshimura E., Mori S. and Nishizawa N.K Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants. Plant Cell Physiol., vol.46 p1809-1818. (2005)§These authors contributed to this work equally)

Ishimaru Y., Suzuki M., Kobayashi T., Takahashi M., Nakanishi H., Mori S., and Nishizawa N.K. OsZIP4, a novel zinc-regulated zinc transporter in rice. J. Exp. Bot., 56: 3207–3214 (2005)

Oki H., Kim S., Nakanishi H., Takahashi M., Yamaguchi H., Mori S. and Nishizawa N.K. Directed evolution of yeast ferric reductase to produce plants with tolerance to iron deficiency in alkaline soils. Soil Sci. Plant Nutr., 50: 1159-1165 (2004)

Koike S., Inoue H., Mizuno D., Takahashi M., Nakanishi H., Mori S., and Nishizawa N.K. OsYSL2 is a rice metal-nicotianamine transporter that is regulated by iron and expressed in the phloem. Plant J., 39: 415-424 (2004)

Takahashi M., Terada Y., Nakai I., Nakanishi H., Yoshimura E., Mori S. and Nishizawa N.K The role of nicotianamine in the intracellular delivery of metals and plant re-productive development. Plant Cell, vol.15 p1263-1280. (2003)

Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S. and Nishizawa N.K. Three rice nicotianamine synthase genes, OsNAS1, OsNAS2 and OsNAS3 are expressed in cells involved in long-distance transport of iron and differentially regulated by iron. Plant J., 36: 366-381. (2003)

Higuchi K., Takahashi M., Nakanishi H., Kawasaki S., Nishizawa N.K. and Mori S. Analysis of transgenic rice containing barley nicotianamine synthase gene. Soil Sci. Plant Nutr., 47: 315-322 (2001)

Higuchi K., Watanabe S., Takahashi M., Kawasaki S., Nakanishi H., Nishizawa N.K. and Mori S. Nicotianamine synthase gene expression differs in barley and rice under Fe-deficient conditions. Plant J., 25: 159-167 (2001)

Takahashi M., Nakanishi H., Kawasaki S., Nishizawa N.K., Mori S. Enhanced tolerance of rice to low iron availability in alkaline soils using barley nicotianamine aminotransferase genes. Nature Biotech. vol.19 p466-469. (2001)

Singh K., Sasakuma T., Bughio N., Takahashi M., Nakanishi H., Yoshimura E., Nishizawa N.K. and Mori S. Ability of ancestral wheat species to secrete Mugineic acid family phytosiderophores in response to iron deficiency. J. Plant Nutr., 23: 1973-1981 (2000)

Takahashi M., Yamaguchi H., Nakanishi H., Shioiri T., Nishizawa N.K., and Mori S. Cloning two genes for nicotianamine aminotransferase, a critical enzyme in iron acquisition (strategy II) in graminaceous Plant. Plant Physiol., 121. p947-956. (1999)

Bughio N., Takahashi M., Yoshimura E., Nishizawa N.K. and Mori S. Light-Dependent Iron Transport into Isolated Barley Chloroplasts. Plant Cell Physiol., 38: 101-105 (1997)

Bughio N., Takahashi M., Yoshimura E., Nishizawa N.K. and Mori S. Characteristics of light-regulated iron transport system in barley chloroplasts. Soil Sci. Plant Nutr., 43: 959-963 (1997)